Using the Micro Sign
The micro sign is the SI prefix for 10⁻⁶, one millionth. It goes in front of a unit symbol with no space: 0.5 µm for a bacterium, 400 µg of folic acid, 20 µs of latency, 47 µF on a capacitor. The prefix itself is spoken "micro", so 5 µL is "five microlitres". It is the only SI prefix that is not a Latin letter, which is exactly why it gets its own page.
Two characters, one shape
Unicode contains both µ (U+00B5 MICRO SIGN) and μ (U+03BC GREEK SMALL LETTER MU). The micro sign was inherited from the Latin-1 character set, where it sat among ° and ± so that typewriter-era documents could show units. The Greek letter is the real mu. They look identical in most fonts, and Unicode treats µ as a compatibility character that decomposes to μ, which means normalised text and many search engines already consider them the same. The practical advice: use µ for units when you type it with a keyboard shortcut, because that is what Alt + 0181 and Option + M produce, and use μ when you are writing Greek or mathematics, such as the mean μ of a distribution or the coefficient of friction. Never mix the two within a document, and when a lab or journal specifies one, follow it.
Common mistakes
The most consequential error is medical: µg (micrograms) mistaken for mg (milligrams) is a thousandfold dose difference, which is why prescribing guidelines in many countries ask for "mcg" or the full word instead of the symbol. In email and spreadsheets people also type "um" or "ug" when the symbol seems out of reach; that is acceptable in a filename but not in a label, a report or a datasheet. Do not use the capital M either, since M means mega, a factor of 10¹².
Typing it
Because µ lives in Latin-1, it has a real Windows Alt code (Alt + 0181) and a Mac shortcut (Option + M), and Word converts 00B5 to µ after Alt + X. In HTML the entity is µ. LaTeX users have several routes: \mu in maths mode gives the Greek letter, while siunitx offers \micro and \si{\micro\meter} for units in text. Phones are the weak point: neither the iPhone keyboard nor Gboard has a long-press for µ, so add the Greek keyboard, which gives you μ on the M key, or set a text replacement.
Beyond the lab
µ also appears in audio (the µ-law codec), in the name of the microkernel and in maker circles, where µC is shorthand for microcontroller. In bios, engineers and photographers use it in handles like "µ4/3" for Micro Four Thirds. Pair it with the ohm symbol and the degree symbol for a full set of unit signs, and see the sigma symbol page for another Greek letter that does double duty in statistics.